"We replaced all of the previous secondaries with Jackson ImmunoResearch Laboratories Inc. antibodies. Good choice of colours/reactivities/hosts. Perform very well in multi-labeling staining due to variety of pre-adsorbed versions available. Very helpful customer service."
Izabela Borek, Medical University of Graz
20-23rd Sep - FENS Regional Meeting - Pecs, Hungary
7-9th Nov - Joint Annual Meetings of the French Society for Immunology and the French Cytometry Association - Reims, France
13-16th Nov - Medica 2017 - Dusseldorf, Germany
Add more colors to multiple labeling assays in the UV/Vis region, with Brilliant Violet 421™ and Brilliant Violet 480™ conjugated secondary antibodies. When combined with Alexa Fluor® 488, Rhodamine-Red™-X, and Alexa Fluor® 647 conjugates, effective 5-color fluorescent labeling is possible.
If nuclear counterstaining is desired, 4-color antibody staining is possible using Brilliant Violet 421, Brilliant Violet 480, Alexa Fluor® 488 and Rhodamine Red™-X. Switching the nuclear stain from DAPI (emission in the blue region) to DRAQ5™ (which has red emission) frees the violet-blue region of the spectrum to accommodate the two Brilliant Violet dyes. DRAQ5’s excitation and emission profiles overlap those of Alexa Fluor 647.
|Excitation Peak (nm)||Emission Peak (nm)|
|Brilliant Violet 421™||407||421|
|Brilliant Violet 480™||436||478|
We offer a range of secondary antibodies that are recommended for multiple labeling due to their minimal cross reactivity, ( See our multiple labeling guidelines for more information).
Available Target Specificities for Multiple Labeling (Designated ML* in product tables)
Excitation and emission curves for BV 421™, BV 480™, Alexa Fluor ® 488, Rhodamine Red™-X, and Alexa Fluor® 657.
DRAQ5 can be used as a nuclear stain in place of Alexa Fluor 647.
BD Brilliant Violet polymer chains can be considered as a collection of optical segments, each with the ability to absorb light and emit fluorescence signal. This results in materials that have a bright fluorescence signal for better resolution and sensitivity.
Light harvesting base polymers have multiple optical segments that can emit for fluorescence detection.
|Channel||Fluorophore||Excitation (nm)||Emission (nm)||Chroma Filter Set|
|Cyan||BV480™||436||478||49302 or 49001|
For more information on these filter sets, please go to the Chroma website.
|Fluorophore||Extinction co-efficient (M -1 cm-1)||Quantum Yield (ɸF)|
Brilliant Violet™ is a trademark of Sirigen Inc., a Becton, Dickinson and Company affiliate.